定量聚合酶連鎖反應 定量聚合酶連鎖反應 (Real-Time Polymerase chain reaction) 目的: 了解定量PCR的原理,並熟悉定量PCR機器的操作流程。 本實驗採用雙股DNA鑲嵌螢光物質Syber-Green I進行絕對定量測試。 基本原理: 於PCR反應液中添加特定的螢光物質,當反應循環數逐次增加 ...
Real-Time PCR 基本原理:. 隨著PCR反應循環逐次增加時,目標DNA也隨之快速增幅,此時. PCR 反應液 .... High Resolution Melt (HRM).
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